NF-κB Luciferase Reporter A549 Cell Line

Product Details

Synonyms	NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb
Description	NF-κB Luciferase Reporter A549 Cell Line is an A549 cell line expressing the NF-κB (nuclear factor kappa0light-chain-enhancer of activated B cells) luciferase reporter. The firefly luciferase reporter is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter. This cell line has been validated for NF-κB stimulation by TNFα (tumor necrosis factor α) and LIGHT (tumor necrosis factor superfamily member 14 or TNFSF14).
Host Cell Line	Human alveolar vassal carcinoma cell line. Adherent epithelial cells.
Applications	• Monitor NF- κB signaling pathway activity. • Screen for compound activity on the NF-κB signaling pathway
Supplied As	Each vial contains ≥1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required For Cell Culture • Thaw Medium 6 • Growth Medium 6B Materials Required for Cellular Assay • hTNFα (carrier free) • LIGHT, His-Tag (Human) Recombinant • IKK-16 dihydrochloride (or other suitable inhibitor control) • Assay Medium: Thaw Medium 6 • Growth Medium 6B • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only