Cat. No.: IBDRGCL60449
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| Synonyms | NF-κB2 p52/p100, NF-κB1 p50/p105, c-Rel, RelA/p65, RelB, nfkb, nf-kb |
| Description | The NF-κB reporter (GFP)-HEK293 cell line is designed for monitoring the nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains the gene for green fluorescent protein (GFP) driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the GFP gene. |
| Supplied As | Two vials containing ~ 1 x 106 cells in 1 mL of 10% DMSO in FBS |
| Materials Required But Not Supplied | • Thaw Medium 1 • TNFα • 96-well tissue culture plate or 96-well tissue culture-treated black clear-bottom assayplate • Fluorometer |
| Mycoplasma Testing | This cell line has been screened using the PCR Based Mycoplasma Detection Kit to confirm the absence of Mycoplasma contamination. |
| Scientific Category | Cell Signaling Pathway |
Storage & Handling
| Storage/Stability | Store in liquid nitrogen immediately upon receipt. Do not store for long-term at -80°C or on dry ice. |
| Shipping Temperature | -80°C |
| Warnings | Avoid freeze/thaw cycles |
| Regulatory Status | Research Use Only |