Myc Luciferase Reporter HCT116 Cell Line

Product Details

Description	The Myc Luciferase Reporter HCT116 Cell Line is a HCT116 cell line expressing firefly luciferase under the control of Myc responsive elements. These cells contain a mutated APC gene, which leads to the aberrant accumulation of β-catenin and constitutive expression of Wnt-responsive genes by the transcription factors TCF/LEF (T cell factor/lymphoid enhancer factor family) and CBP (Creb-binding protein). The transcription factor Myc is an early response gene that is over-expressed as a result of β-catenin signaling in HCT116 cells, leading to constitutive expression of the Myc-dependent luciferase reporter. The cell line is validated for inhibition of Myc activation by ICG-001, an inhibitor of β-catenin/CBP-mediated gene transcription.
Host Cell Line	HCT116, Human Colorectal Carcinoma, epithelial-like cells, adherent
Applications	• Monitor Myc transcriptional activity. • Screen or titrate Myc regulators.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 7 • Growth Medium 7B Materials Used in Cellular Assay • Assay Medium 7B • ICG-001 • 96-well white clear-bottom cell culture plate • Luminometer
UniProt	P01106
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only