Cat. No.: IBDAK-620297
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| Sample Type | Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Homogenate |
| Detection Method | Colorimetric |
| Assay Type | Sandwich (quantitative) |
| Assay Time | 1 h 30 min |
| Assay Duration | One step assay |
| Product Type | Macrophage / Inflamm. |
| Sensitivity | 28 pg/ml |
| Range | 125 pg/ml - 8000 pg/ml |
| Species Reactivity | Reacts with: Mouse |
| Platform | Microplate (12 x 8 well strips) |
Target
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. |
| Sequence Similarities | Belongs to the MIF family. |
| Cellular Localization | Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. |
Storage & Handling
| Storage | Store at -20°C. Please refer to protocols. |