Mouse Leptin R ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard assay
Product Type	Redox Metabolism
Sensitivity	32 pg/ml
Range	32.77 pg/ml - 8000 pg/ml
Species Reactivity	Reacts with: Mouse
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.
Tissue Specificity	Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
Sequence Similarities	Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 4 fibronectin type-III domains. Contains 1 Ig-like (immunoglobulin-like) domain.
Cellular Localization	Secreted and Cell membrane.
Domain	The cytoplasmic domain may be essential for intracellular signal transduction by activation of JAK tyrosine kinase and STATs.The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.