Mouse FAS ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	3 h 30 min
Assay Duration	Multiple steps standard assay
Product Type	Cell Type Markers
Sensitivity	< 3 pg/ml
Range	31.2 pg/ml - 2000 pg/ml
Species Reactivity	Reacts with: Mouse
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue Specificity	Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Sequence Similarities	Contains 1 death domain. Contains 3 TNFR-Cys repeats.
Cellular Localization	Secreted and Cell membrane.
Domain	Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.