Mouse CYR61 ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Cell culture media, EDTA Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Secreted Molecules
Sensitivity	10.1 pg/ml
Range	62.5 pg/ml - 4000 pg/ml
Species Reactivity	Reacts with: Mouse
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Sequence Similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.
Cellular Localization	Secreted.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.