JNK2 ELISA Kit

Overview

Sample Type	Cell culture extracts, Tissue Extracts
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Redox Metabolism
Sensitivity	80.7 pg/ml
Range	234 pg/ml - 15000 pg/ml
Species Reactivity	Reacts with: Mouse, Rat, Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
Sequence Similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.