Cat. No.: IBDRGCL60444
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| Synonyms | JAK reporter, STAT reporter, ISRE cell line |
| Description | The ISRE Reporter – HEK293 Cell Line is designed to monitor the activity of the JAK/STAT signaling pathway. The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. Binding of Interferon alpha (IFNα) to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylates and activates STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex known as ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes. The ISRE Reporter – HEK293 Cell Line contains the firefly luciferase gene under the control of ISRE stably integrated into HEK293 cells. This cell line is validated for the response to stimulation with interferon Alpha and to treatment with JAK inhibitor Pyridone 6. |
| Host Cell Line | HEK293, epithelial-like cells, adherent |
| Applications | • Monitor IFNα-induced activity and JAK/STAT pathway activity. • Screen for compound effect on the JAK/STAT pathway. |
| Supplied As | Each vial contains ~2 X 106 cells in 1 mL of 90% FBS, 10% DMSO |
| Materials Required But Not Supplied | Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1B Materials Required for Cellular Assay • Human Interferon Alpha A (IFNα) • JAK Inhibitor I (Pyridone 6): Prepare stock solution in DMSO. • Assay Medium: Thaw Medium 1 • Growth Medium 1B • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate • Luminometer |
| UniProt | P23458, P29597, P42224, P52630 |
| Mycoplasma Testing | The cell line has been screened to confirm the absence of Mycoplasma species. |
| Scientific Category | Cell Signaling Pathway |
Storage & Handling
| Storage/Stability | Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. |
| Shipping Temperature | -80°C |
| Warnings | Avoid freeze/thaw cycles |
| Regulatory Status | Research Use Only |