In Vitro ADME Services in IBD Drug Discovery

Absorption, Distribution, Metabolism, Excretion (ADME) studies are critical in the development of new drugs for inflammatory bowel disease (IBD). In vitro ADME assays are used to profile the pharmacokinetic properties of a drug candidate, providing essential insight into the metabolism and potential interactions of new IBD drug compounds. As the cost of IBD drug development continues to rise, this testing is essential. In vitro assays in the hit-to-lead and lead optimization phases can reduce failure rates by providing accurate information on ADME, potential drug-drug interactions, and unforeseen toxicities.

Fig. 1. Organs-on-a-chip: current applications and consideration points for in vitro ADME-Tox studies. (Ishida et al., 2018)

Our In Vitro ADME Services

In vitro facilities at Ace Therapeutics provide ADME services for faster and better decision making or as a stand-alone offering to support IBD drug discovery programs. Cost-effective, standardized and customized assays for ADME parameters are provided to clients.

We perform in vitro ADME testing under strictly controlled conditions to help clients gain insight into the DMPK properties of IBD drugs.

• Physicochemical screening: Aqueous solubility (thermodynamic and Kinetic method), Log P (octanol/water), Log D (octanol/PBS).
• Permeability assays: PAMPA, CaCo-2, MDCK (wild type).
• In vitro binding assays: Plasma protein binding (PPB), blood plasma partitioning (BPP).
• Metabolic stability: Plasma stability, blood stability, metabolic stability in liver microsomes, metabolic stability in hepatocytes, intestinal metabolic stability.
• Drug-drug interactions: Cytochrome P450 (CYP) inhibition assay (based on fluorescence and LC-MS/MS).
• Metabolite identification/profiling: Analysis of fecal or urine samples and intestinal tissue can be performed to quantify compound and metabolite levels.

A Global Platform for Your ADME Needs

From effective screening in early discovery to robust regulatory standard study design, our in vitro ADME assays are applicable to both small molecule drugs and biologics of IBD and can be customized to meet the specific requirements of your program.

Our in vitro ADME assays provide critical data on drug properties early in the IBD drug discovery process. This data can help to:

• Guide chemical structure optimization.
• Facilitate ranking and classification of compounds.
• Predict in vivo pharmacokinetic properties and the potential for drug-drug interactions.
• Inform drug optimization during the IBD drug development process.

Ace Therapeutics has the expertise to ensure that your IBD drug's in vitro ADME testing follows the optimal process and regulatory guidelines, while providing the data-driven support you need to design, select and advance your compound to the next milestone. If you are interested in our services, please do not hesitate to contact us.