IL-15 Responsive Luciferase Reporter Cell Line

Product Details

Description	This recombinant Jurkat cell line is a biologically relevant system to measure activation of the IL-15 cytokine receptor by IL-15. The cells were engineered for constitutive expression of human CD122 (IL-15Rβ; IL-2Rβ; NM_000878.4), and conditional expression of firefly luciferase driven by STAT5 response elements located upstream of the minimal TATA promoter. Expression of CD122 allows formation of a functional IL-15 receptor at the surface of Jurkat cells, which naturally express high levels of CD132 (also known as IL-15 receptor subunit γc). Activation of the STAT5 signaling pathway in response to IL-15 or IL-15 analogs can be monitored by measuring luciferase activity.
Host Cell Line	Jurkat cells (clone E6-1), Human T lymphoblast, suspension
Applications	• Characterize recombinant IL-15, IL-15 analogs or other agonists of IL-15R • Screen Janus kinase or IL15R inhibitors in a cell-based assay
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 2 • Growth Medium 2A Materials Required for Cellular Assay • Recombinant Human IL-15 (rhIL-15) • Assay Medium: Thaw Medium 2 • 96-well tissue culture treated, white, clear-bottom assay plate • Luminometer
UniProt	P40933
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only