Human Transferrin Receptor ELISA Kit (CD71)

Overview

Sample Type	Cell culture supernatant, Saliva, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Cell Type Markers
Sensitivity	18 pg/ml
Range	53.13 pg/ml - 3400 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Sequence Similarities	Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain.
Cellular Localization	Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.