Human Toll-like Receptor 9 ELISA Kit

Overview

Sample Type	Cell culture extracts
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Macrophage / Inflamm.
Sensitivity	248 pg/ml
Range	1.25 ng/ml - 80 ng/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue Specificity	Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.
Sequence Similarities	Belongs to the Toll-like receptor family. Contains 26 LRR (leucine-rich) repeats. Contains 1 TIR domain.
Cellular Localization	Endoplasmic reticulum membrane. Endosome. Lysosome. Cytoplasmic vesicle > phagosome. Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.