Human TLR3 ELISA Kit (CD283)

Overview

Sample Type	Cell culture supernatant, Serum, Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (qualitative)
Assay Duration	Multiple steps standard assay
Product Type	Chemokines
Sensitivity	< 10 pg/ml
Range	156 pg/ml - 10000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Microplate

Target

Function	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue Specificity	Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
Sequence Similarities	Belongs to the Toll-like receptor family. Contains 22 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. Contains 1 TIR domain.
Cellular Localization	Endoplasmic reticulum membrane. Endosome membrane.
Domain	ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS