Human NLRP3 ELISA Kit

Overview

Sample Type	Tissue Extracts, Cell Lysate
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Macrophage / Inflamm.
Sensitivity	1.63 ng/ml
Range	4.38 ng/ml - 280 ng/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
Tissue Specificity	Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder.
Sequence Similarities	Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 9 LRR (leucine-rich) repeats. Contains 1 NACHT domain.
Cellular Localization	Cytoplasm.

Storage & Handling

Storage

Store at +4°C. Please refer to protocols.