Human IRAK-1 ELISA Kit

Overview

Sample Type	Cell culture extracts
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	TLR Signaling
Sensitivity	22 pg/ml
Range	156 pg/ml - 10000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Tissue Specificity	Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
Sequence Similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. Contains 1 protein kinase domain.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.