Human IGF2R ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	3 h 30 min
Assay Duration	Multiple steps standard assay
Product Type	Macrophage / Inflamm.
Sensitivity	< 10 pg/ml
Range	156 pg/ml - 10000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Sequence Similarities	Belongs to the MRL1/IGF2R family. Contains 1 fibronectin type-II domain.
Cellular Localization	Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
Domain	Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.