Human HMW Kininogen ELISA Kit, Fluorescent

Overview

Sample Type	Cell culture supernatant, Saliva, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Detection Method	Fluorescent
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Secreted Molecules
Sensitivity	9.5 pg/ml
Range	9.8 pg/ml - 20000 pg/ml
Species Reactivity	Reacts with: Human Does not react with: Cow
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
Tissue Specificity	Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
Sequence Similarities	Contains 3 cystatin domains.
Cellular Localization	Secreted > extracellular space.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.