Human gp130 ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Hep Plasma, Cit plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Cell Type Markers
Sensitivity	24.8 pg/ml
Range	62.5 pg/ml - 4000 pg/ml
Species Reactivity	Reacts with: Human Does not react with: Cow
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue Specificity	Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence Similarities	Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 5 fibronectin type-III domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Cellular Localization	Secreted and Cell membrane.
Domain	The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.