Human Complement C5a ELISA Kit

Overview

Sample Type	Cell culture supernatant, Serum, Plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard assay
Product Type	Macrophage / Inflamm.
Sensitivity	31 pg/ml
Range	31 pg/ml - 2000 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
Sequence Similarities	Contains 1 anaphylatoxin-like domain. Contains 1 NTR domain.
Cellular Localization	Secreted.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	pH: 7.2 Constituent: 100% PBS
Handling	Do Not Freeze.