Human Complement C3b ELISA Kit

Overview

Sample Type	Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	4 h
Assay Duration	Multiple steps standard assay
Product Type	Macrophage / Inflamm.
Sensitivity	80 pg/ml
Range	0.24 ng/ml - 2.5 ng/ml
Species Reactivity	Reacts with: Human
Platform	Microplate

Target

Function	C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Tissue Specificity	Plasma.
Sequence Similarities	Contains 1 anaphylatoxin-like domain. Contains 1 NTR domain.
Cellular Localization	Secreted.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.