Cat. No.: IBDAK-621023
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| Sample Type | Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma |
| Detection Method | Colorimetric |
| Assay Type | Sandwich (quantitative) |
| Assay Time | 1 h 30 min |
| Assay Duration | One step assay |
| Product Type | Cell Type Markers |
| Sensitivity | 9.4 pg/ml |
| Range | 21.88 pg/ml - 1400 pg/ml |
| Species Reactivity | Reacts with: Human |
| Platform | Pre-coated microplate (12 x 8 well strips) |
Target
| Function | Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. |
| Tissue Specificity | Expressed by all cells except erythrocytes. |
| Sequence Similarities | Contains 4 Sushi (CCP/SCR) domains. |
| Cellular Localization | Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide. |
| Domain | Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b. |
Storage & Handling
| Storage | Store at +4°C. Please refer to protocols. |