Human CCL14 ELISA Kit (HCC-1)

Overview

Sample Type	Saliva, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma
Detection Method	Colorimetric
Assay Type	Sandwich (quantitative)
Assay Time	1 h 30 min
Assay Duration	One step assay
Product Type	Chemokines
Sensitivity	4.4 pg/ml
Range	5.5 pg/ml - 350 pg/ml
Species Reactivity	Reacts with: Human
Platform	Pre-coated microplate (12 x 8 well strips)

Target

Function	Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Tissue Specificity	Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
Sequence Similarities	Belongs to the intercrine beta (chemokine CC) family.
Cellular Localization	Secreted.

Storage & Handling

Storage

Store at -20°C. Please refer to protocols.