Hippo Pathway/TEAD Luciferase Reporter MCF7 Cell Line

Product Details

Synonyms	Hippo, Hippo signalling pathway, Hippo signalling cascade, Salvador-Warts-Hippo signalling pathway, SWH signalling pathway
Description	The TEAD Luciferase Reporter MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line MCF7. When the Hippo pathway is not stimulated, transcriptional coactivators YAP (Yes-associated protein) and TAZ (also known as WWTR1, WW domain containing transcription regulator 1) are localized in the nucleus and induce constitutive expression of the luciferase reporter. The cell line has been validated for the inhibition of the luciferase reporter by activators of the Hippo pathway, and for the activation of the luciferase reporter by inhibitors of the Hippo pathway.
Host Cell Line	MCF7 cells, human breast mammary gland cell line, epithelial cells, adherent
Applications	• Monitor Hippo signaling pathway • Screen for activators or inhibitors of the Hippo pathway • Measure the activity of transcription factor TEAD
Supplied As	Each vial contains 2 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1B • Insulin Solution from Bovine Pancreas Materials Used in the Cellular Assay • Thaw Medium 1 • Insulin Solution • Okadaic acid (stock 10 mM in DMSO) • H2O2 • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C (dry ice)
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only