GR-GAL4 Luciferase Reporter Jurkat Cell Line

Product Details

Description	The Glucocorticoid Receptor (GR)-GAL4 Luciferase Reporter Jurkat Cell Line contains an engineered transcription factor stably integrated into the genome of Jurkat cells, which consists of the glucocorticoid receptor ligand binding domain fused to the DNA binding domain of GAL4. This fusion construct activates firefly luciferase expression which is under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	• Monitor glucocorticoid signaling pathway activity. • Screen activators or inhibitors of the glucocorticoid signaling pathway.
Supplied As	Each vial contains 2 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2 • Growth Medium 2A Materials Required for Cellular Assay • Assay Medium 2C  • Dexamethasone • Mifefristone • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only