GR-GAL4 Luciferase Reporter HEK293 Cell Line (Glucocorticoid Receptor Pathway)

Product Details

Synonyms	GAL4, Glucocorticoid Receptor, Galectin
Description	The GR-GAL4 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line expressing firefly luciferase under the control of glucocorticoid receptor ligand binding domain fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression via control of a multimerized GAL4 upstream activation sequence (UAS). This system allows specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and has low cross-reactivity with other nuclear receptor pathways. This cell line has been validated by stimulation with dexamethasone and treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Applications	• Monitor glucocorticoid signaling pathway activity. • Screen for compound activity on the glucocorticoid signaling pathway.
Supplied As	Each vial contains ≥1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1G Materials Required for Cellular Assay • Dexamethasone • Mifepristone/RU-486 • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Biological Activity	This cell line is validated for the response to the stimulation of dexamethansone and to the treatment of the inhibitor mifepristone.
UniProt	P04150
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Immediately upon receipt, store in liquid nitrogen.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only