GIPR/CRE Luciferase Reporter HEK293 Cell Line

Product Details

Synonyms	Gastric inhibitory polypeptide receptor, GIP-R, Glucose-dependent insulinotropic polypeptide receptor, GIPR, GIP responsive cell line, Tirzepatide responsive cell line, Retatrutide responsive cell line
Description	GIPR/CRE Luciferase Reporter HEK293 Cell Line is an engineered HEK293 cell line expressing firefly luciferase under the control of cAMP response element (CRE), and human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity. The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in dose-response assays using the agonists gastric inhibitory peptide (GIP), Tirzepatide, and Retatrutide.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Applications	Screen and characterize agonists of human GIPR in a cellular model.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Gastric Inhibitory Peptide (GIP), human • Tirzepatide hydrochloride • Retatrutide • Opti-MEM Reduced Serum Medium (Assay Medium) • Luminometer
UniProt	P48546
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only