GAS Reporter (Luc)-HeLa Cell Line (IFNγ/JAK/STAT1 Pathway)

Product Details

Synonyms	Gas Cell line, GAS reporter cell line, GAS Hela cell line, STAT1, Just another kinase, JAK, INFγ, Signal transducer and activator of transcription 1, Interferon gamma cell line, JAK pathway bioassay
Description	The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.
Host Cell Line	HeLa
Supplied As	Each vial contains ~2 X 106 cells in 1 mL of 10% DMSO
Materials Required But Not Supplied	• IFNγ • IFNα • Assay medium: Thaw Medium 1 • MEM medium supplemented with 10% FBS, 1% non-essential amino acids , 1 mM Na pyruvate, 1% Penicillin/Streptomycin • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
UniProt	P23458, O60674, P01579, P42224
Mycoplasma Testing	The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Immediately upon receipt, store in liquid nitrogen.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only