Foxp3 Luciferase Reporter Jurkat Cell Line

Product Details

Synonyms	Foxp3, Foxp3 cell line, scurfin cell line, foxo
Description	The forkhead box P3 (Foxp3, also known as scurfin) Luciferase Reporter Jurkat cell line is designed for monitoring the transcriptional activity of the Foxp3 promoter and testing inhibitors that target Foxp3-inducing signaling pathways. It contains a firefly luciferase gene under the control of a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter. This reporter cell line has been tested and validated using phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin. It can be used to measure T cell activation mediated by Dynabeads T-Activator CD3/CD28.
Host Cell Line	Jurkat (clone E6-1), human T lymphoblast, suspension
Applications	Screen for compounds that affect transcription factors that regulate the expression of Foxp3 in a cellular context.
Supplied As	Each vial contains 2 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 2 • Growth Medium 2B Materials Required for Cellular Assay • Assay Medium 2E • Phorbol 12-myristate 13-acetate (PMA) • Ionomycin • Cyclosporin A • Luminometer • 96-well tissue culture-treated white clear-bottom assay plate
UniProt	Q9BZS1
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Immunotherapy

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only