Customized 2D Intestinal Cell Culture Models

The in vitro study of the pathogenesis of inflammatory bowel disease (IBD) requires the use of a cell model demonstrating as closely as possible, the characteristics of the in vivo intestinal epithelium. Most in vitro models have used a single cell type, namely the human intestinal epithelial cell line Caco-2, which has been widely used to study absorptive functions and permeability of the intestinal epithelium. In addition, a number of 2D cell co-culture models have been developed that better mimic the human intestinal epithelium. These intestinal cell culture models are widely used in IBD studies due to their simplicity, reproducibility, and ability to reproduce key aspects of intestinal epithelial biology.

Fig. 1. Schematic overview of the co-culture model with Caco-2 cells and THP-1-cells. (Stevens et al., 2022)

Our 2D Cell Culture Models

Ace Therapeutics offers reliable 2D intestinal cell culture models for preclinical IBD studies. These models provide a simple and robust approach to generating an in vitro intestinal epithelium that provides access to both the luminal and basal cell sides and incorporates critical features required for screening applications.

We can help our clients study intestinal inflammation by single epithelial cells or a combination of epithelial and immune cells. Depending on your research goals, our team of experts will recommend the most appropriate cell model for you. In addition, we can optimize media composition, incubation time, cell density and other parameters for different cell lines to ensure that the cells grow stably and maintain near-physiological properties.

Characterization of 2D Cell Culture Models

We offer a range of in vitro assays to assess the physiological relevance of 2D cell culture models. Our professional team will assist you in analyzing the data, interpreting the meaning of the results, and providing recommendations and guidance for follow-up studies.

• The effect of TNF-α on Caco-2 barrier properties is determined by transepithelial electrical resistance (TEER).
• The immune response of lipopolysaccharides (LPS)-stimulated RAW 246.7 cells is measured by analyzing the release of the cytokines tumor necrosis factor-alpha (TNF)- α interleukin (IL)-6, IL-8, and IL-10 with an enzyme-linked immunosorbent assay (ELISA).

Applications of Our 2D Cell Culture Models

Ace Therapeutics aims to advance clients' preclinical IBD research programs through these models. From model development to drug efficacy evaluation, we provide you with real data.

• Gut inflammation studies
• IBD drug screening
• Drug response analysis
• Testing compounds with anti-inflammatory properties
• New therapeutic target identification
• Potential biomarker identification

With rich experience in cell culture and professional experimental services, Ace Therapeutics provides customized high-quality 2D cell culture models to help you make breakthroughs in your IBD research. Please contact us to learn more about our services.