CSL Reporter – HEK293 Cell line

Product Details

Synonyms	Notch reporter, PKA reporter, cAMP reporter, luciferase reporter , CSL reporter, CBF1 reporter, RBP-J reporter, Cell based assay kit, cell-based assay, reporter assay kit
Description	The Notch CSL Reporter – HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) stably integrated into HEK293 cells. Transfection of this cell line with a Notch expression vector and activation of the Notch pathway leads to expression of luciferase reporter. This cell line is validated with a Notch1DelE expression vector, which is constitutively processed by γ-secretase, leading to the release of NICD and the expression of luciferase.
Supplied As	Each vial contains ~1.5 X 106 cells in 1 mL of FBS with 10% DMSO
Materials Required But Not Supplied	• Assay Medium: Thaw Medium 1 • Growth Medium 1B • Lipofectamine 2000 or other transfection reagent • DAPT : inhibitor of Notch pathway (γ-secretase inhibitor). Prepare 10 mM of stock solution in DMSO • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Immediately upon receipt, store cells in liquid nitrogen.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only