Cat. No.: IBDRGCL60423
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| Synonyms | CRE cell line, CREB cell line, luciferase reporter |
| Description | The CRE/CREB Reporter (Luc) – Jurkat Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into Jurkat cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to stimulation by Forskolin. |
| Host Cell Line | Jurkat |
| Supplied As | Each vial contains ~2 X 106 cells in 1 mL of 10% DMSO |
| Materials Required But Not Supplied | • Forskolin • Assay Medium: Thaw Medium 2 • Growth Medium 2E • 96-well tissue culture treated white clear-bottom assay plate • Luminometer |
| Mycoplasma Testing | The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species. |
| Scientific Category | Cell Signaling Pathway |
Storage & Handling
| Storage/Stability | Immediately upon receipt, store in liquid nitrogen. |
| Shipping Temperature | -80°C |
| Warnings | Avoid freeze/thaw cycles |
| Regulatory Status | Research Use Only |