CRE/CREB Luciferase Reporter HEK293 Cell Line (cAMP/PKA Signaling Pathway)

Product Details

Synonyms	CRE cell line, CREB cell line, luciferase reporter
Description	The CRE/CREB Luciferase Reporter HEK293 Cell Line (cAMP/PKA Signaling Pathway) is an HEK293 cell line that contains a firefly luciferase reporter under the control of multimerized cAMP response element (CRE), designed to monitor the activity of the cAMP/ PKA signaling pathway. Elevation of intracellular cAMP levels activates cAMP response element binding protein (CREB) to bind to CRE and induces expression of luciferase. This cell line is validated for response to stimulation by forskolin and to treatment with an inhibitor of the cAMP/PKA signaling pathway.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Applications	• Monitor cAMP/PKA signaling pathway activity. • Screen for compound activity on the cAMP/PKA signaling pathway
Supplied As	Each vial contains ˃1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required For Cell Culture • Thaw Medium 1 • Growth Medium 1F Materials Required for Cellular Assay • Forskolin: 10 mM in DMSO: activator of cAMP expression • H-89: 10 mM in DMSO: inhibitor of cAMP/PKA pathway • Assay Medium: Thaw Medium 1 • Growth Medium 1F • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only