CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway)

Product Details

Description	The CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway) contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into CHO cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to Forskolin.
Host Cell Line	CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Applications	• Monitor cAMP/PKA signaling pathway activity. • Screen for activators or inhibitors of cAMP/PKA signaling pathway.
Supplied As	Each vial contains 2 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 3 • Growth Medium 3 Materials Required for Cellular Assay • Assay Medium: Thaw Medium 3 • Growth Medium 3 • Forskolin • 96-well tissue culture treated white clear-bottom assay plate • Luminometer
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only