CGRPR/CRE Luciferase Reporter HEK293 Cell Line

Product Details

Synonyms	Calcitonin gene-related peptide type 1 receptor, CGRP type 1 receptor, Calcitonin receptor-like receptor, CRLR, CGRPR, LMPHM8, CALCRL, CLR
Description	Recombinant HEK293 cell line stably expressing full-length human calcitonin receptor-like receptor (CALCRL/CRLR/CLR; accession number: NM_005795) and firefly luciferase under the control of a multimerized cAMP response element (CRE). This cell line can be used to measure the EC50 and IC50 of CGRP receptor agonists and antagonists using luciferase reporter activity as read-out.
Host Cell Line	HEK293, Human Embryonic Kidney, epithelial-like cells, adherent
Applications	• Screen for activators or inhibitors of CGRP receptor for immunotherapy research and drug discovery. • Screen for anti-CGRP antibodies.
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Materials Required for Cell Culture • Thaw Medium 1 • Growth Medium 1G Materials Required for Cellular Assay • Thaw Medium 1 • Growth Medium 1G • MEM medium for preparing assay medium • Bovine Serum Albumin (BSA, protease free) • Human α-CGRP • Human β- CGRP • Rimegepant • Zavegepant (Vazegepant) Hydrochloride • Eptinezumab • Galcanezumab • Anti-CALCRL antibody • PE Donkey Anti-Rabbit Antibody • Luminometer
UniProt	Q16602
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Neuroscience

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only