Assessment of Oxidative Stress Markers in IBD Animal Models

Oxidative stress, among the immune-regulatory factors, has been proposed as one of the major mechanisms involved in the pathophysiology of inflammatory bowel disease (IBD). Chronic intestinal inflammation is associated with overproduction of both reactive oxygen and reactive nitrogen species (ROS and RNS), leading to oxidative and nitrosative stress. Substantial evidence suggests that IBD is associated with an imbalance between ROS and antioxidant activity which creates oxidative stress as the result of either ROS overproduction or a decreased antioxidant activity. A variety of oxidative stress markers have been used as indicators in animal studies of IBD. The evaluation of oxidative stress markers in animal models of IBD is essential to understand the underlying mechanisms of the disease and to develop new therapeutic strategies.

Fig. 1. Detection of indexes about oxidative stress. (Duan et al., 2020)

Our Oxidative Stress Markers Assessment Service

Ace Therapeutics provides reliable oxidative stress marker assessment services to evaluate oxidative damage in animal models of IBD. Our goal is to assist clients in investigating the molecular mechanisms of oxidative stress in IBD and in testing novel redox modulating therapeutics.

We offer a range of tests to analyze the level of oxidative stress in animal models of IBD. Our professional team provides you with high-quality laboratory techniques and data analysis services according to your research needs and requirements, ensuring accurate and reliable results for your studies.

• Measuring ROS levels using electron spin resonance spectroscopy, fluorescence microscopy with specific probes (e.g., dihydroethidium), and flow cytometry.
• Assessing oxidative damage by measuring the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), nitric oxide (NO) synthase (NOS2), lipoxygenases (LOX), and myeloperoxidase (MPO).
• Assessing lipid and protein oxidation by kinetics of catalase (CAT) activity, measurement of protein thiol levels, or antioxidant system status.
• Assessing lipid peroxidation by measuring malondialdehyde (MDA) and conjugated diene levels.
• Detecting the content of ROS using molecular imaging methods for real-time tracking and evaluate the therapeutic effects of drug candidates on mucosal healing in animals with colitis.

Ace Therapeutics is committed to providing clients with excellent scientific support to help pharmaceutical and biotech companies achieve deeper scientific discoveries and breakthroughs. Feel free to contact us to learn more about our oxidative stress marker assessment services!