Assessment of Inflammatory Markers in IBD Animal Models

Chemokines and cytokines play a key role in the regulation of mucosal inflammation by promoting the migration of leukocytes to sites of inflammation, which ultimately leads to tissue damage and destruction. A variety of metrics associated with acute and chronic inflammation are used to assess the severity of the intestinal inflammatory response. Because inflammatory bowel disease (IBD) is difficult to treat, immunotherapy focuses on controlling the severity of the inflammatory response during the activation phase. Studies using IBD patient tissues and animal models of IBD have identified cytokines as potential new targets for the treatment of intestinal inflammation.

Fig. 1. Cytokines in the pathogenesis of IBD. (Neurath, 2014)

Our Inflammatory Marker Assessment Service

As a research service provider in the field of IBD, Ace Therapeutics offers high-quality inflammatory marker assessment services. Our services are focused on helping clients investigate the relationship between the cell products (cytokines and inflammation-related proteins) of immune cells, immunity, and IBD.

We collect the serum, plasma, feces, and colon of IBD animals for subsequent analysis. We have advanced techniques (e.g., enzyme-linked immunosorbent assay ELISA, PCR) to detect and quantify inflammatory markers.

We offer a range of assays to assess the severity of the intestinal inflammatory response and the activation of intestinal neutrophils and macrophages in IBD animals. These assays provide tools to study mechanism of action of various test therapeutics or potential target genes and to explore drug efficacy biomarkers.

• Detecting pro-inflammatory factors such as interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-17 (IL-17), tumor necrosis factor-alpha (TNF-α).
• Detecting reactive proteins and enzymes in serum or plasma, including lysosomal protein myeloperoxidase (MPO), C-reactive protein, anti-neutrophil cytoplasmic antibodies, calprotectin, and chitinase-3-like protein 1.
• Detecting calprotectin, neopterin, neutrophil gelatinase-associated lipocalin, and chitinase-3-like protein 1 in feces or colon tissue.

Ace Therapeutics' inflammatory marker assessment services are designed to help our clients gain a comprehensive understanding of the inflammatory state of IBD animals, providing important data to support research into the mechanisms and therapeutic strategies of IBD. We provide efficient services to ensure client satisfaction and promote the success of your research program. Please feel free to contact us for more information.