ARE Luciferase Reporter HepG2 Cell Line (Nrf2 Antioxidant Pathway)

Product Details

Synonyms	ARE cell line, Nrf2 reporter, Nrf, Hepg2, ARE
Description	The antioxidant response element (ARE) Luciferase Reporter HepG2 hepatic cell line contains a stably integrated firefly luciferase reporter under the control of ARE, which is recognized by the transcription factor Nuclear factor erythroid 2-related factor 2 (Nrf2). Luciferase expression correlates with activation of Nrf2 and the antioxidant pathway. This cell line was validated by stimulation with tert-butylhydroquinone, sulforaphane, and RTA-408 (omaveloxone).
Host Cell Line	HepG2, Human hepatocellular carcinoma, epithelial-like cells, adherent
Applications	• Monitor the Nrf2 antioxidant response pathway. • Screen for compound activity on the Nrf2 antioxidant response pathway
Supplied As	Each vial contains >1 x 106 cells in 1 mL of cell freezing medium
Materials Required But Not Supplied	Media Required for Cell Culture • Thaw Medium 1 • Growth Medium 1k Materials Used in the Cellular Assay • Tert-butylhydroquinone • DL-Sulforaphane • RTA-408 (Omaveloxolone) • White clear-bottom 96-well cell culture plate • Luminometer
UniProt	Q16236
Mycoplasma Testing	The cell line has been screened to confirm the absence of Mycoplasma species.
Scientific Category	Antioxidant Signaling pathway

Storage & Handling

Storage/Stability	Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only