AP-1 Reporter – HEK293 Recombinant Cell Line (JNK signaling pathway)

Product Details

Synonyms	JNK reporter, AP-1 cell line, activator protein-1 cell line, activator protein-1
Description	The stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun.  c-Jun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription. The AP1 Reporter – HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells. This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.
Host Cell Line	HEK293 cells
Applications	• Monitor the JNK signaling pathway activity and AP1-mediated activity. • Screen for activators or inhibitors of the JNK signaling pathway.
Supplied As	Each vial contains ~1.5 X 106 cells in 1 mL of 10% DMSO
Materials Required But Not Supplied	Materials Required but Not Supplied for Cell Culture • Thaw Medium 1 • Growth Medium 1B Materials Required but Not Supplied for Cellular Assay • Phorbol 12-Myristate 13-Acetate (PMA). Prepare stock solution in DMSO. • CC-401 (JNK-401): Prepare stock solution in water • Assay Medium 1B • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate • Luminometer
Biological Activity	This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.
UniProt	P45983, P45984, P53779
Mycoplasma Testing	The cell line has been screened using the PCR-based Mycoplasma Detection kit to confirm the absence of Mycoplasma species.
Scientific Category	Cell Signaling Pathway

Storage & Handling

Storage/Stability	Store in liquid nitrogen immediately upon receipt.
Shipping Temperature	-80°C
Warnings	Avoid freeze/thaw cycles
Regulatory Status	Research Use Only