AMCase Inhibitor Screening Kit (Fluorometric)

Overview

Sample Type	Inhibitor compounds
Detection Method	Fluorescent
Assay Duration	Multiple steps standard assay
Product Type	Macrophage / Inflamm.
Platform	Microplate (12 x 8 well strips)

Target

Function	Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
Tissue Specificity	Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.
Sequence Similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily. Contains 1 chitin-binding type-2 domain.
Cellular Localization	Cytoplasm and Secreted. Secretion depends on EGFR activity.

Storage & Handling

Storage	Store at +4°C.
Storage Buffer	Constituent: 100% PBS