The trabecular meshwork (TM) is a tissue in the eye responsible for regulating aqueous humor (AH) outflow and controlling intraocular pressure (IOP). Since IOP is a major risk factor for glaucoma, TM tissue plays an important role in the pathogenesis of glaucoma. Therefore, understanding the unique proteomic alterations of TM tissue is an important point in the study and prevention of glaucoma.
Fig. 1 Comparison of the trabecular meshwork in normal eyes and glaucoma.
The molecular mechanisms underlying biochemical and functional changes in TM tissues associated with glaucoma have been less studied. Ace Therapeutics’ service aims to enhance the understanding of glaucoma at the molecular level by analyzing the TM proteome of glaucoma models and providing detailed protein molecular information.
TM Proteins Associated with Glaucoma
Dysfunction and lesions of ocular TM tissues often lead to common glaucoma diseases, and therefore TM proteins involved in maintaining the physiological and pathological state of glaucoma have been extensively studied. For example, glucocorticoid response protein/myocilin, fibronectin, optineurin, procollagen C-proteinase enhancer 1 (PCOLCE1), cytochrome P450 1B1, Rho GTPase, etc. have been shown to play a role in the pathogenesis of glaucoma. The identified proteins are involved in biochemical pathways including metabolic processes, cell adhesion, anti-apoptosis, cell motility, carbohydrate metabolic processes, signal transduction and transcriptional regulation.
Services for Proteomic Analysis of Trabecular Meshwork in Glaucoma
- Sample Preparation
To prepare TM tissues from the eyes of individual experimental animals, including animal models of different ages and sexes. Animal species can be selected according to the experiment, such as mouse, rat, pig, etc. All animal experiments for research purposes are performed according to guidelines. In sample preparation, we are able to ensure careful detection and separation of TM tissues and storage at -80°C to ensure sample quality. We also perform a series of pre-processing steps including homogenization of TM tissues, ultrasonication, centrifugation, extraction and etc.
- Proteomics Analysis of TM Tissues
We offer a variety of protein analysis techniques. You can choose from protein assay kits, SDS-PAGE analysis, protein microarrays, LC-MS/MS analysis, immunomes analysis, and other services. For experimental data, we can perform database search identification, GO analysis, protein-protein interaction (PPI) analysis and more analysis to screen for associated proteins.
Our Advantages
- Choosing our systematic research procedure services not only saves research time and effort, but also benefits from the technical expertise and facilities dedicated to large-scale protein and proteomic screening of glaucomatous trabecular networks.
- We have experienced bioscientists in glaucoma and proteomics research to provide our clients with a range of glaucoma-related research and proteomics analysis services.
- We care about your samples from the beginning to the reporting of results. Experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results.
- You can get the industry's most affordable prices and expert advice on experimental design, and talk directly with us about how to meet your specific project requirements.
Our entire service process is simple and flexible, and we are committed to meeting all of your needs. Please feel free to contact us for a quote or to discuss in detail your requirements and how we can support you.
References
- Micera A, et al. Differential Protein Expression Profiles in Glaucomatous Trabecular Meshwork: An Evaluation Study on a Small Primary Open Angle Glaucoma Population. Adv Ther 33, 2016, 252–267.
- Funke S, et al. An In-Depth View of the Porcine Trabecular Meshwork Proteome. Int J Mol Sci, 2019, 20(10):2526.