Quantitative Analysis of Stroke-related Protein and mRNA Expression

The onset of stroke involves many pathophysiological and cellular processes, including edema, cerebral blood barrier disruption, neuroinflammation, immune response, cell death, neuronal remodeling, and spontaneous recovery, etc. Most of the underlying molecular mechanisms are largely unknown, which hinders the identification of new targets and biomarkers and subsequent therapeutic development. Identification and quantification of specific proteins and mRNAs in situ and in brain tissue samples can provide valuable information to elucidate signaling pathways and molecular mechanisms involved in recovery from ischemic brain injury and stroke and aid in the development of therapeutic drugs.

Fig. 1. The expression of TRIM67 decreased after cerebral ischemia-reperfusion injury. (Yu et al., 2023)

Our Services

Ace Therapeutics provides customers worldwide with reliable services to quantify protein and mRNA expression levels in ischemic brain injury, enabling researchers to gain insight into the complex molecular mechanisms of ischemic stroke. The proteins and genes being tested can be selected according to customer needs. The Ace Therapeutics's protein and gene analysis workflow is designed to improve processing efficiency, reproducibility, and result accuracy, helping to provide a reliable data foundation for stroke research in shorter time and simpler operations.

Measurement and Characterization of Stroke-related Protein Expression Levels

We can detect the changes of stroke-related proteins after modeling or after drug administration.

• We provide western blotting (WB), enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry to quantify individual proteins associated with stroke.
• We provide proteomics for the overall characterization of stroke-related protein expression levels, allowing the detection of protein content. By detecting protein expression levels at the histologic level, we help our clients to understand changes in intracellular proteome abundance after stroke ischemia, screen for biomarkers, and explore the effects of proteins on activity, etc.

Detection and Quantification of Stroke-related mRNA Expression Levels

We can detect the changes of stroke-related genes after modeling or after drug administration.

• We can detect genes that are differentially expressed due to stroke using a variety of techniques such as Northern blotting, reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization. Specific genes of interest can be selected and studied according to customer needs.
• We also offer a variety of screening techniques to identify known and unknown differentially expressed genomes in animal models of stroke, including ablation libraries/ablation hybridization, differential hybridization, serial analysis of gene expression, representative difference analysis (RDA), and differential display. Our experts ensure maximum confidence in detecting adaptive upregulation of gene expression.

What We Offer

• Raw data/images
• Data processing results
• Official report

At Ace Therapeutics, we are committed to providing cutting-edge quantitative analysis of stroke-related proteins and mRNAs, enabling researchers to decipher the complexity of stroke pathophysiology and develop effective therapeutic agents. If you are interested in our services, please do not hesitate to contact us!

1. Yu, Y., et al. (2023). TRIM67 alleviates cerebral ischemia-reperfusion injury by protecting neurons and inhibiting neuroinflammation via targeting IκBα for K63-linked polyubiquitination. Cell & Bioscience, 13(1), 99.