Strokes caused by thrombus in the coronary or cerebral arteries are among the leading causes of death and disability worldwide, and there is currently no effective treatment. Animal models of thrombosis play a crucial role in replicating human disease conditions and have been extensively used in the discovery of antithrombotic drugs. Ferric chloride (FeCl3)-induced arterial thrombosis models in different species such as mice, rats and rabbits is particularly suitable for the study of thrombosis.This model involves iron-mediated chemical oxidation, which results in tissue damage that promotes platelet adherence and aggregation, ultimately leading to the activation of coagulation and fibrin formation. It is easily applied to a variety of vessels with different diameters and has a high degree of reproducibility.
Fig. 1 Views of the mouse FeCl3 model of carotid artery thrombosis. (OWENS III, et al., 2011)
Ace Therapeutics provides customized FeCl3-induced arterial thrombosis models to help clients study the pathophysiology of thrombosis, discover and validate novel antithrombotic drugs, and understand the mechanism of action of these agents.
Animals are anesthetized by either parenteral injections (subcutaneous, intraperitoneal, or intravenous) or by inhalation. A midline cervical incision is made, and the left common carotid artery (CCA) is isolated under an operating microscope. A small piece of filter paper soaked in a solution of FeCl3 is placed on the adventitial surface of the carotid artery for several minutes. The size of the filter paper varies from 0.5×1 to 1×2 mm. After removal of the filter paper, the vessel is thoroughly washed with PBS. We offer the flexibility to customize the FeCl3 dose and exposure duration to meet the specific needs of our customers.
We can monitor thrombosis by measuring blood flow at the site of injury using a microvascular ultrasound blood flow probe or intravital microscopy to determine the success of FeCl3-induced arterial thrombosis model.
Ace Therapeutics offers a range of services to help clients evaluate the efficacy of antithrombotic drugs in FeCl3-induced arterial thrombosis models. These services are carefully designed to provide detailed insights into the antithrombotic properties of candidate compounds, enabling clients to make informed decisions throughout their drug development processes.
| Image analysis of thrombus formation |
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| Measurement of thrombus size |
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| Characterization of thrombus composition | Evaluate the effects of pharmacological agents on FeCl3-induced thrombosis in animal models by conducting immunostaining to identify thrombus components, including platelets, erythrocytes, and fibrin. |
| Electron microscopy observation of FeCl3-induced injury of carotid arteries | Identify the spherical bodies after FeCl3 treatment using transmission electron microscopy (TEM) or scanning electron microscopy (SEM). |
Ace Therapeutics provides animal models of arterial thrombosis using FeCl3 as a stimulus, helping clients to evaluate the efficacy of antithrombotic drugs and discover potential therapeutic targets. Our advanced laboratory facilities and experienced scientific team offer robust support to meet the specific needs of each client. If you are interested in our services, please contact us for more information.
Ace Therapeutics is a global leading provider of stroke research services. We are committed to accelerating progress in stroke research and drug development.