Custom Endothelin-1 (ET-1) Induced Ischemic Stroke Model

Endothelin-1 (ET-1) is a peptide with potent and long-lasting vasoconstrictive properties. Application to exposed blood vessels or direct application to the brain surface or stereotaxic injection into the brain parenchyma results in vasoconstriction, causing downstream ischemia. Using this approach, any area in the brain is more or less targetable. Depending on the concentration of ET-1, the severity and duration of ischemia and the resulting infarct size may change. When the effect of ET-1 weakens, blood flow gradually recovers, resulting in transient focal ischemia. Injection of ET-1 near the middle cerebral artery (MCA) results in lesions comparable to those observed using the middle cerebral artery occlusion (MCAO) technique.

Fig. 1. Intracortical injection of endothelin-1 (ET-1) proximal to the calcarine artery results in transient arterial occlusion. (Teo et al., 2014)

Custom ET-1 Induced Ischemic Stroke Model

Ace Therapeutics provides standard operating procedures for stereotactic injection of ET-1 near the MCA in animals. Our ET-1 occlusion modeling service provides a reliable and versatile method to simulate focal ischemic stroke. By utilizing the powerful vasoconstrictive properties of ET-1, we can induce controlled and reversible ischemia in targeted brain regions, helping you to study stroke pathophysiology and evaluate potential therapeutic interventions.

Animal Modeling Method

We can induce ischemic injury by injecting ET-1 into the superficial cortex of conscious rats through a stereotactic cannula adjacent to the MCA.

Animal Model Characterization and Validation

To assess compound efficacy, Ace Therapeutics offers a complete range of predictive outcome scores during stroke as well as neurobehavioral tests for monitoring transient and persistent deficits. Examples of complex behavioral tests can be designed upon request and can be combined with appropriate histology and/or neurochemistry to provide a customized assessment package.

• Behavioral test: We offer assessments of emotional, cognitive, sensorimotor deficits, learning and memory functions
• Ultrasonic vocalization (USV) test: As an add-on to most tests, allowing assessment of communication function and emotional state

Advantages of Our Custom ET-1 Induced Ischemic Stroke Model

• Our process for inducing stroke using ET-1 is relatively simple and easy to perform, with the flexibility to select specific areas of the brain to induce stroke
• In the ET-1 induced ischemic stroke model, we can adjust the dose of ET-1 to control the degree of arterial constriction that induces strokes of varying severity
• Our ET-1 induced ischemic stroke model provides highly reproducible and predictable local brain injury for consistent and standardized experiments
• Our ET-1 induced ischemic stroke model can be used to assess a variety of post-stroke problems, including mood disorders, cognitive deficits, sensorimotor deficits, and learning and memory functions

Applications

• Simulating lacunar stroke
• Long-term studies focusing on stroke recovery mechanisms

Ace Therapeutics guarantees quality data for customizable endothelin-1 (ET-1) induced MCAO model for ischemic stroke. With superior solutions and an experienced team, we are your ideal partner for stroke research. Clients can contact us directly and receive timely feedback.

1. Teo, L., & Bourne, J. A. (2014). A reproducible and translatable model of focal ischemia in the visual cortex of infant and adult marmoset monkeys. Brain pathology, 24(5), 459-474.