Bioluminescence Imaging (BLI) Service in Animal Models of Stroke

In vivo bioluminescence imaging (BLI) involves the noninvasive interrogation of living animals using light emitted by biological reporter cells expressing luciferase. BLI is increasingly being applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular transport, protein-protein interactions. Because of its low cost, high throughput, and relative ease of manipulation in visualizing various in vivo cellular events. BLI has emerged as a powerful tool in the study of animal models of stroke, and bio-optical scene imaging of endogenous and exogenous stem cells for stroke repair allows real-time longitudinal monitoring of neural stem cells in vivo to elucidate their regenerative properties.

Fig. 1. Stroke induces an increase in BLI signal in Nestin-CreERT2/Fluc mice localized to the peri-infarct area. (Vandeputte et al., 2014)

Our Services

At Ace Therapeutics, we offer comprehensive bioluminescence imaging services tailored specifically for stroke animal models to visualize and quantify cells in the brain. We can image the brain in vivo in stroke animals, or we can remove brain tissue and image them in vitro upon request. By recording the same group of subjects at different points in time and tracking the movement and changes of the same target of observation (labeled cells and genes), the data obtained is more realistic and reliable.

We can develop a comprehensive experimental program according to the customer's needs, indicating the sample concentration, dosage, method of administration, the required luciferase gene or fluorescent substance, and other information. We finally feedback to our clients with high-resolution images and analysis reports.

Applications of Our Services

Our bioluminescence imaging services allow us to assist clients in:

• Providing unique information on neural stem cell dynamics, such as location, migration, and proliferation, during a single imaging session in vivo.
• Non-invasive monitoring of inflammatory processes in vivo, facilitating characterization of immune cell dynamics and cytokine expression patterns after stroke.
• Linking luciferase to neural cell-specific promoters to monitor differentiation.
• Studying stroke pathophysiology and identifying suitable targets or testing candidate compounds.

Ace Therapeutics is committed to utilizing highly sensitive luciferase to perform bioluminescence imaging in the brains of living mice to visualize pathophysiological events in rodent stroke models. We aim to accelerate your stroke research. If you are interested in our services, please do not hesitate to contact us!

1. Vandeputte, C., et al. (2014). Bioluminescence imaging of stroke-induced endogenous neural stem cell response. Neurobiology of disease, 69, 144-155.