3D hydrogels are three-dimensional cross-linked networks of hydrophilic polymers that can absorb and retain large amounts of water. In recent years, these hydrogels have received widespread attention for their potential applications in tissue engineering, drug delivery, and biomedical research. These cross-linked polymer hydrogels can replicate the extracellular matrix found in nature.
In the context of the blood-brain barrier (BBB), 3D hydrogels can be used to create 3D models of the BBB in vitro. Researchers can recreate the complex structure and physiology of the BBB in a laboratory setting by using 3D hydrogels as a substrate. Additionally, these hydrogels can be functionalized with specific molecules to mimic the biochemical and biophysical properties of the blood-brain barrier. In Transwell models, for example, researchers can culture traditionally immortalized cells embedded in hydrogels to form multicellular complexes called spheroids. Furthermore, stem cells grown in 3D hydrogels can differentiate into multiple cell types that closely resemble native tissues, such as human iPSC-derived brain microvascular endothelial cells, that can be programmed to form 3D organoid BBB models, which are ideal, reproducible, and scalable in vitro models of the BBB.
The advantage of using 3D hydrogels as BBB model substrates is that they provide a more realistic representation of brain tissue compared to traditional 2D cell culture systems. Furthermore, 3D hydrogel models can form multicellular layers and establish cell-cell interactions, which are crucial for studying the barrier function of the BBB.
Naturally Derived 3D Hydrogels | Synthetic 3D Hydrogels |
Biologically derived natural ECM extracts are the most commonly used 3D hydrogels to simulate physiological extracellular matrix in vitro. Currently, ECM extracts of Engelbreth-Holm-Swarm (EHS) murine sarcoma basement membrane have been widely used in 3D cell culture. | Synthetic 3D hydrogels are matrices for cell culture achieved through cross-linked polymer networks. Chemically defined hydrogel formulations enhance reproducibility compared to native 3D hydrogels, and the presence and concentration of culture medium components (e.g., growth factors) are known. |
Ace Therapeutics provides a variety of naturally derived 3D hydrogels and synthetic 3D hydrogel kits to help you construct an ideal, stable 3D BBB model in vitro that meets your research needs.
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BBM-3C-102 | Collagen Hydrogel (Soft+-Bovine) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Soft+-Bovine) contains purified collagens from the bovine tendon.
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BBM-3C-103 | 3D Cell Culture Hydrogel (100% Peptide) |
3D Cell Culture Hydrogel (100% Peptide) from Ace Therapeutics is a peptide synthetic hydrogel without any animal-derived or bioactive ingredients that can be used as an ideal three-dimensional cell culture scaffold.
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BBM-3C-104 | 3D Cell Culture Scaffold Kit |
3D Cell Culture Scaffold Kit, a ready-to-use hydrogel system, is used for creating a three-dimensional (3D) cell culture environment, which contains proprietary 3D scaffolds for setting up spheroid formations, 3D cell cultures, and pharmacological studies.
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BBM-3C-105 | 3D Cell Culture Matrix Kit (Alginate Hydrogel) |
3D Cell Culture Matrix Kit (Alginate Hydrogel) is used to artificially create a three-dimensional (3D) cell culture environment. The foundation of this kit is alginate, an anionic polysaccharide sourced from algae cell walls.
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BBM-3C-106 | 3D Cell Culture Matrix Kit (BME) |
3D Cell Culture Matrix Kit (BME) is used to artificially create a three-dimensional (3D) cell culture environment. The kit consists of a cell-adhesive matrix with a microporous structure from animal sources.
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BBM-3C-107 | 3D Cell Culture Matrix Kit (Duo-Matrix) |
3D Cell Culture Matrix Kit (Duo-Matrix) is used to artificially create a three-dimensional (3D) cell culture environment, providing a more dynamic microenvironment as opposed to a single component matrix.
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BBM-3C-108 | 3D Cell Culture Hydrogels Kit (Peptides) |
3D Cell Culture Hydrogels Kit (Peptides) is a synthetic peptide hydrogel free of animal origin, which can simulate the extracellular matrix of different tissues for 3D cell cultures.
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BBM-3C-109 | ECM Gel from Engelbreth-Holm-Swarm Murine Sarcoma |
ECM Gel From Engelbreth-Holm-Swarm Murine Sarcoma is prepared from Engelbreth-Holm-Swarm sarcoma in mice for 3D cell culture.
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BBM-3C-110 | ECM Gel from Engelbreth-Holm-Swarm Murine Sarcoma |
ECM Gel From Engelbreth-Holm-Swarm Murine Sarcoma is prepared from Engelbreth-Holm-Swarm sarcoma in mice for 3D cell culture, without phenol red.
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BBM-3C-111 | 3D Cell Culture Gel solution |
3D Cell Culture Gel Solution is a ready-to-use collagen solution that forms a solid gel by heating it to 37°C for cell culture.
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BBM-3C-112 | 3D RGD-CD Hydrogel Kits |
3D Hydrogel Kits (Pre-Configured), a pre-configured 3D hydrogel system, consists of RGD (arginyl glycyl aspartate) degradable polymer and CD cell degradable cross-linking agent. When RGD and CD cross-linkers are mixed, they cross-link into a gel.
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BBM-3C-113 | 3D Hydrogel Kits (ECM) |
3D Hydrogel Kits (ECM) is used to artificially create a three-dimensional (3D) cell culture environment. The kit consists of an extracellular matrix with a microporous structure from human sources.
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BBM-3C-114 | 3D PVA-PEG Hydrogel Kits |
3D Hydrogel Kits (Pre-Configured), a pre-configured 3D hydrogel system, consists of maleimide-functionalized PVA and PEG non-cell-degradable crosslinker. When PVA polymer and CD cross-linkers are mixed, they cross-link into a gel.
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BBM-3C-115 | 3D Cell Culture Hydrogel Matrix |
3D Cell Culture Hydrogel Matrix is a ready-to-use, xeno-free (animal source-free) functional hydrogel for 3D cell culture research.
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BBM-3C-116 | 3D Cell Culture Hydrogel Matrix Kit (RGD) |
3D Cell Culture Hydrogel Matrix Kit (RGD) is a high-concentration, adjustable, xeno-free (animal source-free) hydrogel system. This system was modified with the cell adhesion peptide RGD to promote cell attachment and cell-matrix interactions during 3D cell culture.
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