3D hydrogels are three-dimensional cross-linked networks of hydrophilic polymers that can absorb and retain large amounts of water. In recent years, these hydrogels have received widespread attention for their potential applications in tissue engineering, drug delivery, and biomedical research. These cross-linked polymer hydrogels can replicate the extracellular matrix found in nature.
In the context of the blood-brain barrier (BBB), 3D hydrogels can be used to create 3D models of the BBB in vitro. Researchers can recreate the complex structure and physiology of the BBB in a laboratory setting by using 3D hydrogels as a substrate. Additionally, these hydrogels can be functionalized with specific molecules to mimic the biochemical and biophysical properties of the blood-brain barrier. In Transwell models, for example, researchers can culture traditionally immortalized cells embedded in hydrogels to form multicellular complexes called spheroids. Furthermore, stem cells grown in 3D hydrogels can differentiate into multiple cell types that closely resemble native tissues, such as human iPSC-derived brain microvascular endothelial cells, that can be programmed to form 3D organoid BBB models, which are ideal, reproducible, and scalable in vitro models of the BBB.
The advantage of using 3D hydrogels as BBB model substrates is that they provide a more realistic representation of brain tissue compared to traditional 2D cell culture systems. Furthermore, 3D hydrogel models can form multicellular layers and establish cell-cell interactions, which are crucial for studying the barrier function of the BBB.
Naturally Derived 3D Hydrogels | Synthetic 3D Hydrogels |
Biologically derived natural ECM extracts are the most commonly used 3D hydrogels to simulate physiological extracellular matrix in vitro. Currently, ECM extracts of Engelbreth-Holm-Swarm (EHS) murine sarcoma basement membrane have been widely used in 3D cell culture. | Synthetic 3D hydrogels are matrices for cell culture achieved through cross-linked polymer networks. Chemically defined hydrogel formulations enhance reproducibility compared to native 3D hydrogels, and the presence and concentration of culture medium components (e.g., growth factors) are known. |
Ace Therapeutics provides a variety of naturally derived 3D hydrogels and synthetic 3D hydrogel kits to help you construct an ideal, stable 3D BBB model in vitro that meets your research needs.
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BBM-3C-087 | 3D SG-PVA |
3D SG-PVA consists of polyvinyl alcohol functionalized with thiol-reactive groups (SG-PVA), which can be used together with other components PEG-Link or CD-Link to set up cell-compatible hydrogels.
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BBM-3C-088 | 3D F-Dextran |
3D F-Dextran consists of maleimide-functionalized dextran (Mal-Dextran), which can be used together with other components PEG-Link or CD-Link to set up cell-compatible hydrogels.
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BBM-3C-089 | 3D F-PVA |
3D F-PVA consists of maleimide-functionalized polyvinyl alcohol (Mal-PVA), which can be used together with other components PEG-Link or CD-Link to set up cell-compatible hydrogels.
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BBM-3C-090 | 3D 10x CB-Buffer (pH 5.5) |
3D 10x CB-Buffer (pH 5.5), a buffer solution, provides a cell environment at pH 5.5 for the formation of fast-gelling 3D hydrogels.
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BBM-3C-091 | 3D 10x CB Buffer (pH 7.2) |
3D 10x CB-Buffer (pH 7.2), a buffer solution, provides a cell environment at pH 7.2 for the formation of slow-gelling 3D hydrogels.
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BBM-3C-092 | 3D Cell Culture Kit |
3D Cell Culture Ki is a ready-to-use bioactive gel that simulates the microenvironment and morphological structure of three-dimensional tissues in vivo to provide a three-dimensional microenvironment similar to the in vivo extracellular matrix for cell culture in vitro and in vivo experiments, supporting the three-dimensional growth of various cells.
It does not contain antigenic substances and animal-derived ingredients with no immunogenicity, stable and clear ingredients, a small difference between batches, highly reproducible experiments, and is easy to operate, only needs to operate at RT with no ice required.
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BBM-3C-093 | 3D Cell and Organoid Culturing Gel Kit |
3D Cell and Organoid Culturing Gel Kit is constructed with synthetic biological raw materials to simulate the microenvironment of human tissue. It has stable quality and freely adjustable colloid hardness, easy operation, high culture stability, high cell recovery rate, and high biocompatibility.
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BBM-3C-094 | Collagen Hydrogel (Rat) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Rat) contains purified collagens from rat tendons.
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BBM-3C-095 | Collagen Hydrogel (Soft-Rat) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Soft-Rat) contains purified collagens from rat tendons.
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BBM-3C-096 | Collagen Hydrogel (Soft+-Rat) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Soft+-Rat) contains purified collagens from rat tendons.
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BBM-3C-097 | Collagen Hydrogel (Human) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Human) contains purified collagens from the human tendon.
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BBM-3C-098 | Collagen Hydrogel (Soft-Human) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Soft-Human) contains purified collagens from the human tendon.
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BBM-3C-099 | Collagen Hydrogel (Soft+-Human) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Soft+-Human) contains purified collagens from the human tendon.
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BBM-3C-100 | Collagen Hydrogel (Bovine) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Bovine) contains purified collagens from the bovine tendon.
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BBM-3C-101 | Collagen Hydrogel (Soft-Bovine) |
Collagen Hydrogel from Ace Therapeutics is a biocompatible complex of type I collagen fibers. These products contain a variety of purified, high-quality collagens derived from biological tissues, which facilitate gel formation and help the cultivation of 3D tissue models. When using this type of product for 3D culture, it will not break or tear easily. Collagen Hydrogel (Soft-Bovine) contains purified collagens from the bovine tendon.
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