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- BBB DamageCategories
- Neurotransmitter Antibodies & Receptor Antibodies
- Ionotropic Receptor AntibodiesMetabotropic Receptor AntibodiesNeurotransmitter AntibodiesTransporter AntibodiesSignal Channel Antibodies
- Calcium Channel AntibodiesChloride Channel AntibodiesDense-Core Vesicles AntibodiesNitric Oxide Channel AntibodiesStroke Pathology Antibodies
- Neurogenesis AntibodiesNeuronal Growth & Development AntibodiesNeuronal Growth & Development AntibodiesNeuronal Metabolism AntibodiesSignal Transduction AntibodiesStroke-Related Cell Markers Antibodies
- Glial Cell Marker AntibodiesNeural Stem Cell Marker AntibodiesNeuron Marker AntibodiesSynaptic Marker AntibodiesCell Death and ApoptosisNeuroinflammationNeuronal Damage and RepairOxidative StressVascular Mechanisms
- 3D BBB Models
- Organ-on-a-Chip BBB ModelsTranswell Models3D BBB Models Culture Substrate
- 3D HydrogelsCollagensAnmial BBB ModelsCell Lines for BBB Modeling
- Immortalized CellsPrimary CellsStem Cells
3D BBB Models Culture Substrate
The BBB is a specialized barrier in the brain that regulates the entry of molecules and cells from the bloodstream into the brain. It plays a crucial role in protecting the brain from harmful substances and maintaining its homeostasis.
Traditional 2D cell model of BBB culture is performed on simple, nonporous two-dimensional (2D) surfaces. Despite the method being easy and convenient, can result in certain well-documented artifacts. The 3D BBB model culture substrate refers to the three-dimensional culture system used for culturing and studying the blood-brain barrier (BBB) model in vitro, which aims to mimic the physiological conditions of the brain's microenvironment, allowing researchers to study BBB function and dysfunction in a controlled laboratory setting. It typically consists of a specialized culture substrate that supports the growth and differentiation of brain endothelial cells, which form the main component of the BBB. The substrate is often coated with extracellular matrix proteins like collagen or laminin to provide a suitable surface for cell attachment and growth.
A well-established method for culturing 3D BBB models is to culture cells on structural scaffolds, which are typically composed of biopolymers, such as 3D hydrogels and collagens, arranged to mimic the physiological extracellular matrix (ECM).
Highlighted below are the types of brackets Ace Therapeutics offers for 3D BBB model culture.
Click on the Links to Access Our 3D BBB Model Culture Substrate:
Cat.# Name Description Price BBM-3C-056 Porcine Gelatine Porcine Gelatine from Ace Therapeutics is a purified gelatin matrix from porcine tissue. The product can enhance the adsorption of various normal cells and transfected cells.inquiry BBM-3C-057 Collagen Solution, 3.0 mg/mL Collagen Solution, 3.0 mg/mL from Ace Therapeutics is purified from bovine tissue. The solution is used to prepare collagen-coated cell culture surfaces to promote adhesion, growth, morphology, migration, and differentiation of various cell types. This product is suitable for culturing human hematopoietic stem cells.inquiry BBM-3C-058 Rat Tail Tendon Collagen Type I, 5mg/ml Rat Tail Tendon Collagen Type I, 5mg/ml is prepared by the method of Birkedal-Hansen through acetic acid extraction, sodium chloride precipitation, disodium hydrogen phosphate precipitation, and other steps. This product can be used to coat cell culture vessels and culture some cells that are difficult to adhere to in ordinary cell culture vessels. It can also be used to prepare three-dimensional glue to simulate a real growth environment and allow cells to grow in a three-dimensional environment.inquiry BBM-3C-059 Collagens Type I-A Collagens Type I-A is acid-soluble Type-I collagen derived from porcine tendon. This product can quickly form a gel and is suitable for 3D cell culture products. It has high gel strength and transparency and is easy to observe under the microscope.inquiry BBM-3C-060 Collagens Type I-P Collagens Type I-P is pepsin-solubilized Type-I collagen derived from pig tendon. The product has low viscosity, making it easy to handle and form gels.inquiry BBM-3C-061 Collagens Type I-C Collagens Type I-P is pepsin-solubilized Type-I collagen derived from pig skin. This product is suitable for coating.inquiry BBM-3C-062 Collagens Type Ⅳ Collagens Type IV is derived from bovine tissue treated with pepsin. This product does not gel and needs to be mixed with collagen type I-A.inquiry BBM-3C-063 Collagen Gel Culturing Kit Collagen Gel Culturing Kit from Ace Therapeutics is a ready-to-use kit for form gel. This kit consists of the all reagents required for the experiment, including cellmatrix type I-A, Ham F-12 culture medium, MEM Hanks culture medium, and reconstitution buffer.inquiry BBM-3C-064 Collagen, Type I, from Rat Tail The rat tail collagen I provided by Ace Therapeutics is prepared according to the Birkedal-Hansen method through acetic acid extraction, sodium chloride precipitation, and disodium hydrogen phosphate precipitation. This product is derived from a rat tail, dissolved in 0.02M HAc solution, and the purity is greater than 90% as determined by SDS-PAGE, and it is sterile.inquiry BBM-3C-065 Collagen, Type I, from Mouse Tail The mouse tail collagen I provided by Ace Therapeutics is prepared according to the Birkedal-Hansen method through acetic acid extraction, sodium chloride precipitation, and disodium hydrogen phosphate precipitation. This product is derived from a mouse tail, dissolved in 0.02M HAc solution, and the purity is greater than 90% as determined by SDS-PAGE, and it is sterile.inquiry BBM-3C-066 Collagen Type I, from Rat Tail The rat tail collagen I provided by Ace Therapeutics is prepared according to the Birkedal-Hansen method through acetic acid extraction, sodium chloride precipitation, and disodium hydrogen phosphate precipitation. This product is a sterile solution dissolved in 6mM HAc, with a concentration of 5mg/ml. Each batch of products passes cell culture testing (including three-dimensional space culture) to ensure quality reliability. Note: Please keep the entire operation at a low temperature on ice because rat tail collagen I at room temperature can quickly form a gel.inquiry BBM-3C-067 Cultrex 3-D Culture Matrix Rat Collagen I Cultrex 3-D Culture Matrix Collagen I from Ace Therapeutics is purified from rat tail tendon with high concentrations. This product is capable of polymerizing to form hydrogels and is a purified matrix ECM protein specifically designed and developed for 3D culture.inquiry BBM-3C-068 Cultrex Mouse Collagen IV Cultrex Mouse Collagen IV from Ace Therapeutics is a stromal extracellular matrix protein purified from a murine engelbreth-holm-swarm (EHS) tumor. This product can promote cell adhesion in cell and tissue cultures as well as be used as a hydrogel or thin coating for tissue culture vessels.inquiry BBM-3C-069 Cultrex Rat Collagen I Cultrex Rat Collagen I from Ace Therapeutics is a high concentration matrix extracellular matrix protein purified from rat tail tendon. This product is capable of polymerizing to form hydrogels and is a purified matrix ECM protein specifically designed and developed for 3D culture.inquiry BBM-3C-070 Cultrex Bovine Collagen I Cultrex Bovine Collagen I from Ace Therapeutics is a purified stromal extracellular matrix protein from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. This product promotes cell adhesion for cell and tissue culture.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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