3D BBB Models Culture Substrate

The BBB is a specialized barrier in the brain that regulates the entry of molecules and cells from the bloodstream into the brain. It plays a crucial role in protecting the brain from harmful substances and maintaining its homeostasis.

Traditional 2D cell model of BBB culture is performed on simple, nonporous two-dimensional (2D) surfaces. Despite the method being easy and convenient, can result in certain well-documented artifacts. The 3D BBB model culture substrate refers to the three-dimensional culture system used for culturing and studying the blood-brain barrier (BBB) model in vitro, which aims to mimic the physiological conditions of the brain's microenvironment, allowing researchers to study BBB function and dysfunction in a controlled laboratory setting. It typically consists of a specialized culture substrate that supports the growth and differentiation of brain endothelial cells, which form the main component of the BBB. The substrate is often coated with extracellular matrix proteins like collagen or laminin to provide a suitable surface for cell attachment and growth.

A well-established method for culturing 3D BBB models is to culture cells on structural scaffolds, which are typically composed of biopolymers, such as 3D hydrogels and collagens, arranged to mimic the physiological extracellular matrix (ECM).

Highlighted below are the types of brackets Ace Therapeutics offers for 3D BBB model culture.

Click on the Links to Access Our 3D BBB Model Culture Substrate:

Collagens 3D Hydrogels

Cat.#	Name	Description	Price
BBM-3C-041	Human Collagen Type IV	Human Collagen Type IV from Human Cell Culture from Ace Therapeutics is derived from human placenta.	inquiry
BBM-3C-042	Collagen Type IV (Human Placenta)	Collagen Type IV (Human Placenta) from Human Cell Culture from Ace Therapeutics is derived from human placenta and is highly purified native, type IV collagen.	inquiry
BBM-3C-043	Collagen from Human Placenta	Collagen from Human Placenta is used to induce differentiation of endothelial cells and rat pluripotent cells. This product is a lyophilized powder that can be reconstituted at 1 mg/mL in sterile 0.5 M acetic acid, PBS, or water.	inquiry
BBM-3C-044	Collagen, Type I Solution from Rat Tail	Collagen, Type I Solution from Rat Tail from Ace Therapeutics is derived from rat tail, which used in tissue engineering especially in the generation of 3-D scaffolds based gels.	inquiry
BBM-3C-045	Collagen from Human Placenta	Collagen from Human Placenta is supplied as a 0.5 M acetic acid solution. It can be used to coat tissue culture surfaces to promote cell attachment	inquiry
BBM-3C-046	Rat Tail Collagen I, 3 mg/mL	Rat Tail Collagen I, 3 mg/mL from Ace Therapeutics is derived from rat tail, which can be prepared as a clear gel to provide a 3D matrix or surface coating on tissue culture plates as a substrate for culturing primary cells such as keratinocytes and hepatocytes.	inquiry
BBM-3C-047	Rat Tail Collagen Type I, Sterile, Filtered	Rat Tail Collagen Type I, Sterile, Filtered, from Ace Therapeutics is a purified collagen derived from the rat tail. The Product can be used as a thin layer on tissue culture surfaces to enhance cell adhesion and proliferation, or as a glue layer to promote the expression of specific cell morphology and function. The product is typically used to culture endothelial cells, stem cells, muscle cells, and a variety of other cells.	inquiry
BBM-3C-048	Rat Tail Collagen Type I, High Concentration, Sterile, Filtered	Rat Tail Collagen Type I, High Concentration, Sterile, Filtered, from Ace Therapeutics is a highly concentration, purified collagen derived from the rat tail. This product is more suitable for 3D cell culture due to the high concentration improves matrix stiffness and support integrity.	inquiry
BBM-3C-049	Human Collagen Type I	Human Collagen Type I from Ace Therapeutics is a purified collagen derived from adult human placenta tissue, and this product can be used as a thin layer on tissue culture surfaces to enhance cell adhesion and valorization.	inquiry
BBM-3C-050	Bovine Collagen Type I	Bovine Collagen Type I from Ace Therapeutics is a purified collagen derived from bovine tissue. The native collagen sequence contained in the preparation is missing or shortened since this product has been treated with pepsin.	inquiry
BBM-3C-051	Human Collagen Type III	Collagen Type III is found in a variety of conjunctival tissues, including human skin, and the cornea. Human Collagen Type III from Ace Therapeutics is a purified collagen derived from adult human placenta tissue, and the product can be used as a coating on tissue culture surfaces to promote cell adhesion and regulate cell behavior.	inquiry
BBM-3C-052	Human Collagen Type IV	Collagen Type IV is a common component of the basement membrane. Human Collagen Type IV from Ace Therapeutics is a purified collagen derived from adult human placenta tissue, and the product can be used to regulate cell growth, differentiation, and tissue formation.	inquiry
BBM-3C-053	Mouse Collagen Type IV	Collagen Type IV is a common component of the basement membrane. Mouse Collagen Type IV from Ace Therapeutics is a purified collagen derived from murine engelbreth-holm-swarm (EHS) tumor, and the product can be used to regulate cell growth, differentiation, and tissue formation.	inquiry
BBM-3C-054	Human Collagen Type V	Collagen Type V is found in the placenta, amniotic membrane, and cornea. Human Collagen Type V from Ace Therapeutics is a purified collagen from adult human placenta tissue. This product can be used as a coating on tissue culture surfaces to study the effects of collagen on cell behavior.	inquiry
BBM-3C-055	Human Collagen Type VI	Collagen Type VI is a multi-domain ECM. Its heterotrimeric chains pack into a microfibrillar network through tetramerization and tail-end linkage. Human Collagen Type VI from Ace Therapeutics is a purified collagen from adult placenta tissue. This product is usually used for coating and can also be added to cell culture fluid.	inquiry

All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.