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- BBB DamageCell Death and ApoptosisNeuroinflammationNeuronal Damage and RepairOxidative StressVascular Mechanisms
- 3D BBB Models
- Organ-on-a-Chip BBB ModelsTranswell Models3D BBB Models Culture Substrate
- 3D HydrogelsCollagensAnmial BBB ModelsCell Lines for BBB Modeling
- Immortalized CellsPrimary CellsStem Cells
3D BBB Models Culture Substrate
The BBB is a specialized barrier in the brain that regulates the entry of molecules and cells from the bloodstream into the brain. It plays a crucial role in protecting the brain from harmful substances and maintaining its homeostasis.
Traditional 2D cell model of BBB culture is performed on simple, nonporous two-dimensional (2D) surfaces. Despite the method being easy and convenient, can result in certain well-documented artifacts. The 3D BBB model culture substrate refers to the three-dimensional culture system used for culturing and studying the blood-brain barrier (BBB) model in vitro, which aims to mimic the physiological conditions of the brain's microenvironment, allowing researchers to study BBB function and dysfunction in a controlled laboratory setting. It typically consists of a specialized culture substrate that supports the growth and differentiation of brain endothelial cells, which form the main component of the BBB. The substrate is often coated with extracellular matrix proteins like collagen or laminin to provide a suitable surface for cell attachment and growth.
A well-established method for culturing 3D BBB models is to culture cells on structural scaffolds, which are typically composed of biopolymers, such as 3D hydrogels and collagens, arranged to mimic the physiological extracellular matrix (ECM).
Highlighted below are the types of brackets Ace Therapeutics offers for 3D BBB model culture.
Click on the Links to Access Our 3D BBB Model Culture Substrate:
Cat.# Name Description Price BBM-3C-072 Ready-to-use 3D Hydrogel kit Ready-to-Use 3D Hydrogel Kit is a set of reagents for constructing extracellular matrix components in 3D cell culture, and is similar to a living extracellular matrix. The kits contain reagents for the preparation of hydrogels with a soft stiffness and an RGD peptide concentration of 0.4 mmol/L. You can use these reagents to control the extracellular environment and design 3D systems suitable for cell survival.inquiry BBM-3C-073 3D F-Dextran-PEG Hydrogel Kit 3D Dextran-PEG Hydroge Kit contains the necessary reagents for creating hydrogels that gel fast and are compatible with living cells, which are maleimide functionalized dextran (Mal-Dextran), and PEG-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on PEG-Link with maleimide groups on the Mal-Dextran, when the two reagents are combined.inquiry BBM-3C-074 3D F-Dextran-CD Hydrogel Kit 3D Dextran-CD Hydrogel Kit contains the necessary reagents for creating hydrogels that gel fast and are compatible with living cells, which are maleimide functionalized dextran (Mal-Dextran), and CD-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on CD-Link with maleimide groups on the Mal-Dextran, when the two reagents are combined.inquiry BBM-3C-075 3D F-PVA-PEG Hydrogel Kit 3D PVA-PEG Hydrogel Kit contains the necessary reagents for creating hydrogels that gel fast and are compatible with living cells, which are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and PEG-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on PEG-Link with maleimide groups on Mal-PVA, when the two reagents are combined.inquiry BBM-3C-076 3D F-PVA-CD Hydrogel Kit 3D PVA-CD Hydrogel Kit contains the necessary reagents for creating hydrogels that gel fast and are compatible with living cells, which are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and CD-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on CD-Link with maleimide groups on Mal-PVA, when the two reagents are combined.inquiry BBM-3C-077 3D S-Dextran-PEG Hydrogel Kit 3D Dextran-PEG Hydroge Kit contains the necessary reagents for creating hydrogels that gel slowly and are compatible with living cells, which are SG-Dextran and PEG-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on PEG-Link with thiol-reactive groups on SG-Dextran, when the two reagents are combined at physiological pH (pH 7.2).inquiry BBM-3C-078 3D S-Dextran-CD Hydrogel Kit 3D Dextran-CD Hydrogel Kit contains the necessary reagents for creating hydrogels that gel slowly and are compatible with living cells, which are SG-Dextran and CD-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on CD-Link with thiol-reactive groups on SG-Dextran, when the two reagents are combined at physiological pH (pH 7.2).inquiry BBM-3C-079 3D S-Life PVA-PEG Hydrogel Kit 3D PVA-PEG Hydrogel Kit contains the necessary reagents for creating hydrogels that gel slowly and are compatible with living cells, which are the polymeric SG-PVA and PEG-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on PEG-Link with thiol-reactive groups on SG-PVA, when the two reagents are combined at physiological pH (pH 7.2).inquiry BBM-3C-080 3D S-Life PVA-CD Hydrogel SG Kit 3D PVA-CD Hydrogel Kit contains the necessary reagents for creating hydrogels that gel slowly and are compatible with living cells, which are polymeric SG-PVA and CD-Link, a cross-linking agent. The formation of a gel through the stable bonding of thiol groups on CD-Link with thiol-reactive groups on SG-PVA, when the two reagents are combined at physiological pH (pH 7.2).inquiry BBM-3C-081 3D Dextran-HA Hydrogel Kit 3D Dextran-HA Hydrogel Kit contains the necessary reagents for creating hydrogels containing hyaluronic acid, which are SG-Dextran and HyLink, a thiol-functionalized hyaluronic acid crosslinker. The formation of a gel through the stable bonding of thiol groups on HyLink with thiol-reactive groups on SG-Dextran, when the two reagents are combined at physiological pH (pH 7.2).inquiry BBM-3C-082 3D PVA-HA Hydrogel Kit 3D PVA-HA Hydrogel Kit contains the necessary reagents for creating hydrogels containing hyaluronic acid, which are SG-PVA and HyLink, a thiol-functionalized hyaluronic acid crosslinker. The formation of a gel through the stable bonding of thiol groups on HyLink with thiol-reactive groups on SG-PVA, when the two reagents are combined at physiological pH (pH 7.2).inquiry BBM-3C-083 3D PEG-Link 3D PEG-Link molecule contains polyethylene glycol that has a thiol at each end. When it is mixed with polymers, such as Mal-PVA, Mal-Dextran, SG-PVA, and SG-Dextran, the thiol groups on PEG-Link create stable thioether bonds with thiol-reactive groups on the polymers, leading to the formation of the hydrogel.inquiry BBM-3C-084 3D CD-Link When the 3D CD-Link molecule, which is made up of a polyethylene glycol-peptide conjugate with thiol groups on both ends, is mixed with polymers Mal-PVA, Mal-Dextran, SG-PVA, and SG-Dextran, the thiol groups on CD-Link react with thiol-reactive groups on the polymers, forming stable thioether bonds, thus leading to the creation of the hydrogel.inquiry BBM-3C-085 3D HyLink 3D HyLink molecule consists of hyaluronic acid carrying thiol groups along its backbone. When combined with polymers SG-PVA and SG-Dextran, thiol groups on HyLink form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel.inquiry BBM-3C-086 3D SG-Dextran 3D SG-Dextran consists of dextran functionalized with thiol-reactive groups (SG-Dextran), which can be used together with other components PEG-Link or CD-Link to set up cell-compatible hydrogels.inquiry All of our products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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